Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

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Effect of essential oil of Cymbopogan caseius and Cymbopogan nardus against Aflatoxin producing Aspergillus flavus

Harish Chandra

High Altitude Plant Physiology Research Centre, H. N. B. Garhwal University (A Central University), Srinagar, Garhwal, Uttarakhand.

Abstract

Effect of individual and combined essential oils of Cymbopogan caesius and C. nardus on growth of aflatoxin producing fungus Aspergillus flavus and aflatoxin production was investigated in vitro. Different concentrations (50 ppm, 100 ppm, 200 ppm and 300 ppm) of essential oils significantly inhibited fungal growth and total aflatoxin content in Mycological broth as compared to that of control. Combinations of essential oils had synergistic inhibition of fungal growth and aflatoxin production as compared to their individual oil. Inhibition of fungal growth in combined essential oils ranged from 37.4% to 100% with an average of 75.7%, respectively. The results of the present study suggest that the synergistic effect of plant extracts can be used for control of fungal growth and aflatoxin production.

Antifungal, Antiaflatoxigenic, C. caesius, C. nardus

Ansari, M.A. and Razdan, R.K., 1995. Relative efficacy of various oils in repelling mosquitoes. Indian J. Malariol., 32: 104–111.

Azzouz, M. A. and Bullerman, L. B., 1982. Comparative antimycotic effects selected herbs, Spices, Plant components and commercial antifungal agents. J.  Food Prot., 45: 1298 – 1301.

Bahk, J. and Marth, E. H., 1983. Aflatoxin production is inhibited by selected herbal drugs. Mycopath., 8: 129 –134.

Bard, M., Albrecht, M.R, Gupta, N., Gyynn, C.J. and Stiliwell, W. (1988). Geraniol interference with membrane function in strains of Candida and Saccharomyces. Lipids. 23: 534-538.

Bauer, A.W, Kirby, W.M.M., Shomi, J.C. and Turck, M., 1966. Antibiotic susceptibility testing by standardized single disc method. Am. J. Clin. Path., 45: 493-496.

Bullerman, L. B., Lieu, F. Y and Seier, S. A. 1977. Inhibition of growth and aflatoxin production by cinnamon and clove oils, cinnamic aldehyde and Eugenol. J. Food Sci.,42: 1107 – 1109.

Delespaul, Q., de Billerbeck, V.G., Roques, C.G. and Michel, O., 2000. The antifungal activity of essential oils as determined by different screening methods. J. Essent. Oil Res. 12:256–266.

Ganjewala, D., 2009. Cymbopogan essential oil: Chemical compositions and biological activities. Int. J Essen. Oil Thera.3:56-65.

Hitokoto, H., Morozumi, S., Wanke, T., Sakai, S. and Kurata, H., 1980. Inhibitory effects of spice on growth and toxin production of toxigenic fungi. Appl. Env. Microbiol. 39: 818 –822

IARC (International Agency for Research on Cancer)., 1993. IARC Monograph on the Evaluation of Carcinogenic Risk to Humans, vol. 56. IARC, Lyon, France.

Jagadish Chandra, K.S., 1975. Recent studies on Cymbopogon Spreng. (Aromatic grasses) with special reference to Indian taxa: Taxonomy, Cytogenetics, Chemistry and Scope. J. Plantation Crops. 3(2): 1-5, 43-57.

Jiujiang, Y., Bhatnagar, D. and Ehrlich, K.C., 2002. Aflatoxin biosynthesis. Revista Iberoamericana de Micologia., 19: 191–200.

Kumar, A., Shukla, R., Singh, P., and Dubey, N. K., 2009. Biodeterioration of some herbal raw materials by storage fungi and aflatoxin and assessment of Cymbopogon flexuosus essential oil and its components as antifungal. Int. Biodete Biodeg., 63(6): 712-716.

Leontopoulos, D., Siafaka, A. and Markaki, P., 2003. Black olives as substrate for Aspergillus parasiticus growth and aflatoxin B1 production. Food Microbiol., 20 (1): 119–126.

Lewis, L., Onsongo, M., Njapau, H., Schurz-Rogers, H., Luber, G., Kieszak, S., Nyamongo, J., Backer, L., Dahiye, A.M., Misore, A., DeCock, K. and Rubin, C., 2005. Aflatoxin contamination of commercial maize products during an outbreak of acute aflatoxicosis in eastern and central Kenya. Environ. Health Persp. 113:1763–1767.

Mahdavi, B., Yaacob, W.A., Din,  L. B. and Siti Aisha, M.A. (2013).Essential Oil Composition of Three Air-Dried Parts of Etlingera brevilabrum. Journal of Essential Oil Bearing Plant.16 (1): 17 – 22

Mallavarapu, G.R., Rao, B.R.R., Kaul, P.N., Ramesh, S. and Bhattacharya, A.K., 1998. Volatile constituents of the essential oils of the seeds and the herb of palmarosa (Cymbopogon martinii (Roxb.) Wats. var. motia Burk.). Flav. Fra.  J. 13:167–169.

Molyneux, R.J., Mahoney, N., Kim, J.H. and Campbell, B.C., 2007. Mycotoxins in edible tree nuts. Int. J. Food Microbiol., 119:72–78.

Morozumi, S. (1978). Isolation, Purification and antibiotic activity of O-Methoxycinnm aldehydes from cinnamon. Appl.  Environ. Microbiol.,  36: 577-583.

Namazi, M., Allameh, A., Aminshahidi, M., Nohee, A. and Malekzadeh, F., 2002. Inhibitory effects of ammonia solution on growth and aflatoxin production by Aspergillus parasiticus NRRL-2999. Acta Poloniae Toxicol., 10(1):65–72.

Oyebangi, A.O. and Efiuvwevwere, B.J.O., 1999. Growth of spoilage mold and aflatoxin B1 production in naturally contaminated or artificially inoculated maize as influenced by moisture content under ambient tropical condition. Int.  J. Biodet. Biodeg. 44:209-217.

Payne, G.A., 1998. Process of contamination by aflatoxin-producing fungi and their impact on crops In: Sinha, K.K.S., Bhatnagar, D. (Eds.), Mycotoxins in Agriculture and Food Safety. Marcel Dekker, Inc., New York, pp. 279–306.

Pittet, A., 1998. Natural occurrence of mycotoxins in foods and feeds – an updated review. Rev. Med. Vet., 149:479–492.

Rasooli, I. and Abyaneh, M.R., 2004. Inhibitory effect of Thymes oil on the growth and Aflatoxin production by Aspergillus parasiticus. Food Control. 15: 479-483.

R-Biopharm GmbH. 2005. Enzyme immunoassay for the quantitative analysis of aflatoxin. Art. No.: R4701. R-Biopharm GmbH, Darmstadt, Germany.

R-Biopharm GmbH. 2005. Immunoaffinity column for sample clean up prior to analysis of aflatoxin. Art. No.: 5001/R5002. R-Biopharm GmbH, Darmstadt, Germany.

Sidhu, O.P., Chandra, H. and Behl, H.M., 2009. Occurrence of aflatoxins in mahua (Madhuca indica Gmel.) seeds: Synergistic effect of plant extracts on inhibition of Aspergillus flavus growth and aflatoxin production. Food Chem. Toxicol.,  47(4):774-777

Smith, J.E. and  Moss, M.O., 1985. Control of Mycotoxins. John Wiley, pp. 127–142.

Thanaboripat, D., Mongkontanawut, N., Suvathi, Y. and Ruangrattanametee, V., 2004. Inhibition of aflatoxin production and growth of Aspergillus flavus by citronella oil, KMITL Sci. J., 4(1):1-8.

Viollon, C. and Chaumont, J.P.1994. Antifungal properties of essential oils and their main components upon Cryptococcusneoformans. Mycopathologia 128: 151–153.

Yin, M. C. and Cheng, W. S., 1998. Inhibition of Aspergillus niger and Aspergillus flavus by some herbs and spices. J. Food Prot., 61: 123-125.

Harish, C. (2016). Effect of essential oil of Cymbopogan caseius and Cymbopogan nardus against aflatoxin producing Aspergillus flavus. Environment Conservation Journal17(3), 109-114.

https://doi.org/10.36953/ECJ.2016.17321

Received: 21.08.2016

Accepted: 19.11.2016

First Online: 18.12. 2016

https://doi.org/10.36953/ECJ.2016.17321

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Publisher Name:  Action for Sustainable Efficacious Development and Awareness (ASEA)

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