{"id":2924,"date":"2020-02-11T19:33:47","date_gmt":"2020-02-11T14:03:47","guid":{"rendered":"https:\/\/environcj.in\/?page_id=2924"},"modified":"2020-05-30T19:52:34","modified_gmt":"2020-05-30T14:22:34","slug":"volume-18-issue-3-18315","status":"publish","type":"page","link":"https:\/\/environcj.in\/volume-18-issue-3-18315\/","title":{"rendered":"volume-18-issue-3\/18315"},"content":{"rendered":"\t\t
Microbial diversity of Gumki cave and their potential role in enzyme production<\/strong><\/span><\/p> Rachna Rautela<\/strong>\u00a0, Seema Rawat, Rashmi Rawat, Pramila Verma, \u00a0A.B.Bhatt<\/strong><\/span><\/p> Department of Botany and Microbiology, Hemwati Nandan Bahuguna Garhwal University, Srinagar, India<\/span><\/strong>\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t The work presents microbial community structure of Gumki cave for bioactive compound production. This cave represents a unique atmosphere which is totally different from outer atmosphere. Total 49 isolates were recovered from cave samples. Morphological and biochemical characterization revealed a community that contains nine genera of bacteria: Bacillus (27%), Paenibacillus (21%), Staphylococcus (20%), Streptococcus (16%), Salimicrobium (8%), Lysisnibacillus (2%), Aeromonas (2%), Proteus (2%) and Clostridium (2%). All these microbes were screened for different enzyme production and about 90% isolates displayed positive results for these enzymes. 75.51% recovered isolates were lipase producers, 47% were producing amylase and 24% and 12% bacteria produced protease and cellulase, respectively.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t Amylase, Bacterial diversity, Cave, Cellulase, lipase, Protease<\/em><\/strong><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t Bakri, Y., Ammouneh, H., El-Khouri, S., Harba, M. and\u00a0 Thonart, P., 2012. Isolation and identification of a new Bacillus <\/em>strain for amylase production. Research in Biotechnology<\/em><\/strong>; 3(6): 51-58.<\/p> Banerjee, S. and Joshi, S. R., 2013. Insights into cave architecture and the role of bacterial biofilm. Proc Natl Acad Sci India B Biol Sci<\/strong><\/em>; 83(3):277\u201390.<\/p> Baskar, S., Baskar, R. L., Mauclaire, L. and\u00a0 McKenzie, A.\u00a0 J., 2006. 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P. de., Montoya, Q. V., Rodrigues, A., Bichuette, M. E., and Seleghim, M.H.R., 2016. Terrestrial filamentous fungi from Gruta do Cata\u02dco (Sa\u02dco Desiderio, Bahia, Northeastern Brazil) show high levels of cellulose degradation.\u00a0 J Caves Karst Stud<\/strong>,<\/em> 78(3); 208\u2013217.<\/p> Shah,\u00a0 K. R. and\u00a0 Bhatt,\u00a0 S. A., 2011. Purification and characterization of lipase from Bacillus subtilis<\/em> Pa2 . J Biochem Tech,<\/em><\/strong> 3:292\u20135. 30.\u00a0<\/p> Soares, I.,\u00a0 Ta\u0301vora, Z., Barcelos, R. P. and\u00a0 Baroni, S., 2012. Microorganism-Produced Enzymes in the Food Industry, Scientific, Health and Social Aspects of the Food Industry, Dr. Benjamin Valdez (Ed.), ISBN<\/em>: 978-953-307-916-5.<\/p> Teather, R.M. and Wood, P.J., 1982. Use of congo red polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl.Environ. Microbio,<\/em><\/strong> 43: 777-780.<\/p> Tomova1, I., Lazarkevich, I., Tomova, A., Kambourova, M. and Vasileva-Tonkova, E., 2013. Diversity and biosynthetic potential of culturable aerobic heterotrophic bacteria isolated from Magura Cave, Bulgaria. Int J Speleol<\/em><\/strong>, 42(1): 65-67.<\/p> Yang, J., Kharbanda, P. D. and Mirza, M., 2004. Evaluation of\u00a0 Paenibacillus polymyxa<\/em> pkb1 for biocontrol of Pythium disease of cucumber in a hydroponic system. Acta Horticulturae,<\/em><\/strong> 635: 59-66.<\/p> Yun, Y., Xiang,\u00a0 X., Wang,\u00a0 H., Man, B., Gong, L., Liu, Q., Dong Q and\u00a0 Wang R. 2016. Five-year monitoring of bacterial communities in dripping water from the Heshang cave in Central China: Implication for\u00a0 paleoclimate reconstruction and ecological functions, . Geomicrobiol J<\/em><\/strong>, 33(7): 1-11.<\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t Rautela, R., Rawat, S., Rawat, R., Verma, P., & Bhatt, A. B. (2017). Microbial diversity of Gumki cave and their potential role in enzyme production.\u00a0Environment Conservation Journal<\/i>,\u00a018<\/i>(3), 115-122.<\/p> :https:\/\/doi.org\/10.36953\/ECJ.2017.18315<\/a><\/strong><\/span><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t This work is licensed under a\u00a0Creative Commons Attribution-Non Commercial- 4.0 International License (CC BY- NC 4.0)<\/strong><\/a>\u00a0<\/p> \u00a9 ASEA<\/strong><\/p><\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\tAbstract<\/span><\/h1>
Chem. Eng. Trans.<\/em><\/strong>38: 277-282.<\/p>