Molecular resolution of some West African Birds using DNA Barcoding
Echi, P.C , Suresh, K. U, George S, Ratheesh R.V
Rajiv Gandhi Center for Biotechnology, Thiruvananthapuram, India
I. M, Ejere, V. C, Eyo, and Nwani, C.D
Department of Zoology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Ezeonu, JE
Department of Microbiology, University of Nigeria, Nsukka, Enugu State, Nigeria.
Abstract
DNA barcoding is widely used for specie cataloging for unambiguous identification and conservation. In the present study, baseline information on the DNA barcoding of mitochondrial CO1 gene of some randomly caught West African birds were provided with five newly reported sequences for Tringa sp, Streptopelia decipens, Francolinus bicalcaratus, Tyto alba and Francolinus squamatus. Species identifications were made by comparing unknown CO1sequences against the DNA barcodes of known species through distance-based tree construction and alignment searching. The newly generated sequences are deposited in GenBank under accession numbers JX160000 – JX160017 and JX220905. Current study may contribute towards the development of a reference DNA barcoding database of West African birds.
Conservation, COI sequence, DNA reference library, mtDNA, West African birds
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This work is licensed under a Creative Commons Attribution-Non Commercial- 4.0 International License (CC BY- NC 4.0)
© ASEA
Received: 29.05.2015
Revised: 11.10.2014
Accepted: 02.11.2014
First Online: 12.06. 2015
Publisher Name: Action for Sustainable Efficacious Development and Awareness (ASEA)
Print : 0972-3099
Online :2278-5124