Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

Environment
Conservation Journal

"An International Journal Devoted to Conservation of Environment"

(A PEER REVIEWED JOURNAL)

ISSN: 2278-5124 (Online) :: ISSN: 0972-3099 (Print)

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Effect of sucrose on in-vitro pollen germination of Gloriosa superba L. – a medicinal plant from the Garhwal Himalaya, India.

P. Tiwari, Ekta Chaudhary

Department of Botany and Microbiology, HNB Garhwal University Srinagar (Garhwal) 246174, Uttarakhand, India

P. L. Uniyal 

Department of Botany, University of Delhi 110007, Delhi India

Abstract

Pollen germination and pollen tube growth are prerequisites for fertilization in seed bearing plants. The present study deals with the morphology, production, pollen-ovule ratio, and effect of sucrose on in-vitro germination of pollen grains of Gloriosa superba L., family Colchicaceae. The G. superba L. pollen were radially symmetrical, prolate shape, 2-3 zonicolpate with striate surface. A single anther produced an average of 350,000 pollen grains. Pollen-ovule ratio was 58333.33/1. The maximum pollen germination (98.86%±0.641) along with 676.2µm pollen tube growth was observed in 5% sucrose solution, while in 20% sucrose concentration the germination percent decreased by 90% and the pollen grains’ cytoplasm also shrinked. The pollen viability was found 96.22% and 91.79% in IKI and acetocarmine stain respectively. The study is useful in determining the pollen production and potential pollination capacity of Gloriosa superba L., as it is found that the species is xenogamous (self-compatible). The findings will be useful in assessing the pollen vigour by monitoring the rate of germination over a period of time on the length of pollen tubes.

Gloriosa superba L., Pollen morphology, Pollen/ovule ratio, Pollen fertility, Sucrose effect, Garhwal Himalaya.

Abhishek, M.,  Satish, K.V., Santosh, K. S., Deepika, M., Prasad GBKS. and Dua, V.K. 2011. Investigation of anti-inflammatory properties of Swertia chirayta and Gloriosa Superba, Recent Research in Science and Technology, 3(3): 40-43.

Anandhi, S., Rajamani, K., Jawaharlal, M., Maheshwaran, M. and Gnanam, R. 2013. Interspecific hybridization in glory lily. Am. Int. J. of Res. in Formal, Applied & Natu. Sci., 13-135.

Badola, H.K. 2002. Endangered medicinal plant species in Himachal Pradesh. Curr. Sci., 83:797-798.

Bhattacharya, A. and Mandal, S. 2000. Pollination biology in Bombax ceiba Linn. Curr. Sci., 79:1706-1712.

Brewbaker, J.L. and Kwack, B.H. 1963 The essential role of calcium ion in pollen germination and pollen tube growth. Am J Bot., 50:747-758.

Chopra,  R.N., Nayar, S.L. and Chopra, I.C. (1956) Glossary of Indian medicinal plants. 125-126.

Cruden, R.W. 1977. Pollen-ovule ratios: a conservative indicator of breeding systems in flowering plants. Evolution, 31:32–46.

Dafni, A. and Firmage, D. 2000. Pollen viability and longevity practical, ecological and evolutionary implications. Plant System. Evol.222:113-132.

Erdtmann, G. 1952. Pollen morphology and Plant Taxonomy. Angiosperms. Chron. Bot. Co., Waltham Mass, P. 539.

Gaur, R.D. 1999. Flora of the District Garhwal North West Himalaya (With EthnobotanicalNotes) Transmedia: Srinagar Garhwal, India.

Gupta, L.M. and Raina, R. 2001. Significance of sequential opening of flowers in Gloriosa superba L. Curr. Sci.,80(10):1266-1267.

Gupta, L.M., Rana, R.C., Raina, R. and Gupta, M. 2005. Colchicine content in Gloriosa superba L. J. of Research, 4: 238-241.

Katara, M.M. 2013. In-vitro pollen germination of Datura metal L. ( 5 ) Effect of hormones. I. J. of Scientific Research 2(12): 51-52.

Ma, L.G., Fan, Q.S., Yu, Z.Q., Zhou, H.L., Zhang, F.Z. and Sun, D.Y. 2000. Does aluminium inhibit pollen germination via extracellular calmodulin. Plant cell Physiol., 41(3): 372-376.

Mamtha, H. 1989  Studies on the problem of fruit set and its improvement in Gloriosa superba L. M.Sc. (Hort) Thesis, University of Agricultural Sciences, Bangalore.

Mamatha, H., Farooqi, AA., Joshi, SS. and Prasad, T.G. 1992. Pollen studies in Gloriosa superba L. Crop Research,  5(1): 92-97.

Marcellán, O.N. and Camadro, E.L. 1996. The viability of Asparagus pollen after storage at low temperatures. Scientia Horticulturae , 67: 101-104.

Mautinho, A., Camacho, L., Haley, A., Pais, M.S., Trewavas, A. and Malho, R. 2001. Antisense perturbation of protein function in living pollen tubes. Sexual Plant Reproduction, 14: 101-104.

Megha, D.M. and Subrata, M. 2014. Studies on in vitro pollen germination of Phyllanthus reticulatus poir  Indian Journal of Fundamental and Applied Life Sciences,  4 (3): 367-373.

Padmapriya, S., Rajamani, K. and Sathiyamurthy, V.A. 2015. Glory Lily (Gloriosa superba L.) – A Review  I. J. of Current Pharmaceutical Review and Research, 7(1): 43-49.

Pan, R.C., Wang, X.J. and Li, N.H. 2009. “Plant Physiology Higher Education Press, Beijing,  p. 36.

Rajamani, K., Chitra, R., Padmapriya, P., Kumanan, K. and Vadivel, E. 2009. Gloriosa taxonomy, pharmacology and crop husbandry. International journal of Agricultural Enviromental and Biotechnology, 2 (4):341-354.

Rehana, B. and Nagarajan, N. 2011. Antibacterial potential of glory lily, Gloriosa Superba Linn, International Research Journal Of Pharmacy, 2(3): 139-142.

Shivanna, K.R. and Johri, B.M. 1985. The angiosperm pollen: structure and function. Wiley Eastern, New Delhi.

Tarar, J.L. and Vishwakarma, M. 1995. Chromosome of diploid and tetraploid Gloriosa superba Linn. In: Padhye, M.D., Mukherjee, P.K. and Khalatkar, A.S. (Editors). Botany towards 2000 A.D. (Prof. V.R. Dnyansagar Commemoration Volume).Vedams Book, New Delhi, India.

Tuinstra, M.R. and Wedel, J. 2000. Estimation of pollen viability in grain sorghum. Crop Science Madison, 40: 968–970.

Wang, Z.Y., Ge, Y., Scott, M. and Spangenberg, G. 2004. Viability and longevity of pollen from transgenic and nontransgenic tall fescue (Festuca arundinacea) (Poaceae) plants. American Journal of Botany91(4):523-530.

Zhang, T. & Huang, M. 2009 The effect of sucrose and PEG on the in-vitro Germination of Camellia Pollen. Northern Horticulture1: 101-102.

Tiwari, P., Uniyal, P. L., & Ekta, C. (2017). Effect of sucrose on in-vitro pollen germination of Gloriosa superba L.-a medicinal plant from the Garhwal Himalaya, India. Environment Conservation Journal18(1/2), 183-187.

:https://doi.org/10.36953/ECJ.2017.181225

Received: 05.01.2016

Revised: 25.02.2016

Accepted: 12.03.2017

First Online: 15.06. 2017

:https://doi.org/10.36953/ECJ.2017.181225

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