Optimization of various physio-chemical parameters to enhance production of secondary metabolite from soil actinomycetes against dermatophytes
Jadon P. , Dwivedi A., Singh C., Kumar A
Department of life sciences ITM University Gwalior (M.P.)
Abstract
The main objective of the study is to isolates actinomycetes from the Gwalior region and improves the production of secondary metabolite and cell growth of actinomycetes. Total 70 actinomycetes were isolated and screened for maximum secondary metabolites production against the dermatophytes on the bases of agar diffusion method & disc diffusion method. Out of 70 isolate, AP-27 has a good potential to produced secondary metabolite that inhibit the growth of dermatophytes (Microsporum gypseum, Microsporum fulvum, Tricophyton rubrum, and Tricophyton mentagrophytes). The optimization of several growth parameters like- growth medium, Incubation time, carbon sources, nitrogen sources, temperature, pH and minerals for the maximum production of secondary metabolites were checked. The optimum growth of AP-27 occurred with starch casein broth containing 2% starch as carbon source and nitrogen source was potassium nitrate nitrate at 2% at pH 7 and temperature 30⁰C. Soybean meal was also found to be the best nitrogen source after the potassium nitrate which can help in large scale production because In India 74% of soyabean is produced by Madhya Pradesh. FeSO4 (0.01gm/100ml) was selected as optimum mineral source. It was noticed that 6 days old culture was showing the maximum zone of inhibition and cell growth.
Actinomycetes, Dermatophytes, optimization, secondary metabolites
Bundale, S., Begde, D., Nashikkar, N., Kadam, T., and Upadhyay, A., 2015, “Optimization of Culture Conditions for Production of Bioactive Metabolites by Streptomyces spp. Isolated from Soil”, Advance In Microbiology, 5: 441-451.
Dahiya, N., Tewari, R., and Hoondal, G.S., 2006, “Biotechnological aspects of chitinolytic enzymes”, Applied Microbiology and Biotechnology, 71: 773-782.
Devi, N.K.A., Jeyarani, M., and Balakrishnan, K., 2008, “Isolation and identification of marine actinomycetes and their potential in microbial activity”, Pakistan Journal of Biological Sciences, 9(3): 470–472.
Doddamani, P.V., Harshan, K.H., Kanta, R.C., Gangane, R., and Sunil K.B., 2013, “Isolation,Identification and Prevelance of Dermatophytes in Tertairy Care Hospital in Gulbarga District”, People’s journal of Scientific research, 6(2): 10-13.
Gebreel, H.M., El-Mehalawy, A.A., El-Kholy, I.M., Rifaat, H.M., and Humid, A.A., 2008, “Antimicrobial activities of certain bacteria isolated from Egyptian soil against pathogenic fungi” Research Journal of Agriculture and Biological Sciences, 4(4): 331-339.
Jyotsnamayee, S., and Gupta, N., 2010 “Nutritional factors affecting the antifungal activity of Penicillium steckii of mangrove origin”, African Journal of Microbiology Research, 4(3): 126-135.
Kavitha, A., Vijayalakshmi, M., Sudhakar, P. and Narasimha, G., 2010, Screening of Actinomycete strains for the production of antifungal metabolites. African Journal of Microbiology Research, 4(1): 27-32.
Kumar, S., and Kannabira, K., 2012, Cytotoxicity and antioxidant activity of 5-(2,4-dimethylbenzyl)pyrrolidin-2-one extracted from marine Streptomyces VITSVK5 spp, Saudi Journal of Biological Sciences, 19: 81–86.
Kumari, M., Myagmarjav, B. E., Prasad, B. and Choudhary, M. 2013. Identification and characterization of antibiotic-producing actinomycetes isolates. American Journal of Microbiology, 4(1): 24-31.
Majumdar, M., and Majumdar, S., 1965, “Effects of Minerals on Neomycin Production by Streptomyces fradiae”, Journal of Applied Microbiology, 13: 190-193.
Mangamuri, U., Muvva,V., Poda, S.,Nagagani, K., Munaganti, R.K., Chitturi, B., and Yenamandra, V., 2016, “Bioactive metabolites produced by Streptomyces Cheonanensis VUK-A from Coringa mangrove sediments: isolation, structure elucidation and bioactivity”, 3 Biotech, 6:63-70.
Parmar, P., Ojha, V.P., and Subramanian, R.B., 2010, “Optimization of Fusaric acid production by Fusarium oxysporum f. Sp. lycopersici using response surface methodology”, International Journal of Science and Technology, 3(4): 411-416.
Shukla, S., Shukla, H., and Pandey, A.K., 2014, “Optimization of various parameters for production of antimicrobial compounds by fusarium roseum”, World journal of Pharmacy and Pharmaceutical Science, 3(12): 890-905.
Song, Q., Yun H.Y., Hui, and Yang, H.,2012, “Optimization of Fermentation Conditions for Antibiotic Production by Actinomycetes YJ1 Strain against Sclerotinia sclerotiorum”, Journal of Agriculture Sciences, 4(7): 95-102.
Jadon, P., Dwivedi, A., Singh, C., & Kumar, A. (2019). Optimization of various physiochemical parameters to enhance production of secondary metabolite from soil actinomycetes against dermatophytes. Environment Conservation Journal, 20(1&2), 35-40.
:
This work is licensed under a Creative Commons Attribution-Non Commercial- 4.0 International License (CC BY- NC 4.0)
© ASEA
Received: 29.09.2018
Revised: 28.11.2018
Accepted: 14.01.2019
First Online: 20.06. 2019
Publisher Name: Action for Sustainable Efficacious Development and Awareness (ASEA)
Print : 0972-3099
Online :2278-5124